Coding

Part:BBa_K5349001:Design

Designed by: Tomoharu Sakata   Group: iGEM24_BotchanLab-Tokyo   (2024-09-26)


Coupled Translation of scpA (methylmalonyl-CoA mutase) and argK (membrane-bound ATP kinase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2879
    Illegal BamHI site found at 983
    Illegal BamHI site found at 1179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 513


Design Notes

The DNA was synthesized using the Integrated DNA Technologies (IDT) DNA synthesis service. Additionally, certain codons were modified to reduce synthesis difficulty while maintaining the original sequence's functionality.


Source

The sequence data was obtained from the Escherichia coli K12 MG1655 genome available in the National Center for Biotechnology Information (NCBI) database.

References